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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: PKR, a cognitive decline biomarker, can regulate translation via two consecutive molecular targets p53 and Redd1 in lymphocytes of AD patients
doi: 10.1111/j.1582-4934.2009.00688.x
Figure Lengend Snippet: RT‐PCR analysis of p53 gene in lymphocytes of control individuals and Alzheimer patients. Representative agarose gel of p53 gene expression was shown in lymphocytes of four individuals and four Alzheimer, alternatively. The specific bands at 292 pb and 528 pb correspond to p53 and GAPDH genes, respectively. The bands were quantified by using Geldoc software. The results were expressed as arbitrary units and the line represented the mean of 39 controls and 46 Alzheimer patients. *P < 0.05 statistical difference from control individuals (Mann‐Whitney’s test). ▾ Control individuals, ♦ Alzheimer patients.
Article Snippet: Then, signals were captured and analysed under UV with
Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Agarose Gel Electrophoresis, Gene Expression, Software
Journal: Journal of Cellular and Molecular Medicine
Article Title: PKR, a cognitive decline biomarker, can regulate translation via two consecutive molecular targets p53 and Redd1 in lymphocytes of AD patients
doi: 10.1111/j.1582-4934.2009.00688.x
Figure Lengend Snippet: RT‐PCR analysis of Redd1 gene in lymphocytes of control individuals and Alzheimer patients. Representative gel of Redd1 gene expression was shown in lymphocytes of four individuals and four Alzheimer, alternatively (A). The specific bands of 721 pb and 528 pb are Redd1 and GAPDH genes, respectively. The bands were quantified by using Geldoc software and the results were expressed as arbitrary units (A). Line represented the mean of 41 control and 51 Alzheimer patients. **P < 0.01 statistical difference from control individuals (Mann Whitney’s test). (B) Western blot analysis of Redd1 expression in lymphocytes of 37 control individuals and 52 Alzheimer patients. Three isoforms of Redd1 (75, 65 and 53 kD) were detected with the polyclonal goat antibody against Redd1 and actin was shown at 42 kD. The bands were quantified by using Genetools software. Line represented the mean (B). *P < 0.05 statistical difference from control individuals (Mann Whitney’s test). ▾ Control individuals, ♦ Alzheimer patients.
Article Snippet: Then, signals were captured and analysed under UV with
Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Gene Expression, Software, Western Blot, Expressing